Environmental enteric dysfunction (EED) is an intestinal disorder common among children living in low-resource settings. EED is associated with increased risk of growth stunting, cognitive deficits, and reduced responsiveness to oral vaccines. This resource provides an overview of PATH's work to develop a prototype assay to quantitate multiple markers of EED, systemic inflammation, growth hormone (GH) resistance, and micronutrients for use in EED clinical research in low-resource settings.