The project ‘Accelerating access and uptake of HIV self-testing (HIVST) in India’ was launched in February 2020 to demonstrate the feasibility and acceptability of HIVST among different key populations (KPs) and other high-risk groups. The overall goal was to increase the demand for and access to HIVST, as well as subsequent treatment and prevention services, thereby contributing to further reduction in the number of new HIV infections and averting deaths due to HIV infection. This report details the approach, methodology, findings, and recommendations from the study.