We describe the functional elements of a diagnostic instrument and disposable enteric card system under development that rapidly identifies and differentiates Shigella, E. coli, Campylobacter, and Salmonella in stool samples. These elements, currently realized as microfluidic subcircuits on individual cards, are: (1) pathogen capture and lysing, (2) nucleic acid capture, (3) on-chip rapid PCR, and (4) lateral flow detection of amplicons. Presented at the Solid State Sensor, Actuator and Microsystems Workshop, June 4, 2006; Hilton Head Island, SC.
Author(s): Weigl BH, Gerdes JC, Tarr P, et al
Corporate author(s): PATH
Publication date: July 2006
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