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Optimizing Direct Membrane and Direct Skin Feeding Assays for Plasmodium falciparum Transmission-Blocking Vaccine Trials in Bancoumana, Mali

This article, published in The American Journal of Tropical Medicine and Hygiene, reports the results of a study conducted in Mali that identified and optimized parameters that contribute to successful mosquito feeding outcomes in both direct skin feeds (DSFs) and direct membrane feeding assays (DMFA). These assays are critical tools in detecting parasite transmission in a standardized manner, and thus, in developing transmission-blocking malaria vaccines. The study found that younger mosquitoes were significantly associated with higher feeding, survival, and infection rates; other variables such as membrane type and location of the mosquito bite did not significantly affect infection outcome. The article provides specific recommendations for optimal practices for DSFs and DMFAs to further transmission-blocking malaria vaccine development. ABSTRACT ONLY. (Learn how users in developing countries can gain free access to journal articles.)

Author: Coulibaly MB, Gabriel EE, Sinaba Y, et al.

Published: 2017

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(Located at www.ajtmh.org)

Citation: Coulibaly MB, Gabriel EE, Sinaba Y, et al. Optimizing Direct Membrane and Direct Skin Feeding Assays for Plasmodium falciparum Transmission-Blocking Vaccine Trials in Bancoumana, Mali. The American Journal of Tropical Medicine and Hygiene. 2017;Early Online Publication.

Resource types: Peer-reviewed journal

Diseases/vaccines: Malaria

Topics: Disease/vaccine specific information

Regions: Africa