PATH | VRL
Menu

A Multi-Laboratory Study of Diverse RSV Neutralization Assays Indicates Feasibility for Harmonization With an International Standard

This article, published in Vaccine, assesses the current level of agreement between 12 virus neutralization assay formats used in the evaluation of vaccine candidates for respiratory syncytial virus (RSV), a leading cause of lower respiratory tract infection globally. In order to assess the feasibility for assay output harmonization using a reference standard, twelve laboratories participated in this blinded survey study, each using their existing RSV neutralization assay method on a blinded and randomized specimen panel. The results of an independent statistical analysis of the dataset indicate that while precision was consistently high, agreement among the assays varied widely. Based on their findings, the authors recommend establishment of an international standard to improve harmonization of assay results. The study results provide information on the suitability of samples for developing an international standard.

Author: Hosken N, Plikaytis B, Trujillo C, Mahmood K, Higgins D, the Participating Laboratories Working Group

Published: 2017

» Visit web page (English)

(Located at www.sciencedirect.com)

Citation: Hosken N, Plikaytis B, Trujillo C, Mahmood K, Higgins D, the Participating Laboratories Working Group. A Multi-Laboratory Study of Diverse RSV Neutralization Assays Indicates Feasibility for Harmonization With an International Standard. Vaccine. 2017;35(23):3082-3088.

Resource types: Peer-reviewed journal

Diseases/vaccines: Respiratory syncytial virus (RSV)

Topics: Disease/vaccine specific information

Regions: Global