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Quantitative PCR for Detection of Shigella Improves Ascertainment of Shigella Burden in Children With Moderate-to-Severe Diarrhea in Low-Income Countries

This article, published in the Journal of Clinical Microbiology, reports on a study to estimate the prevalence of Shigella by using a new quantitative PCR (qPCR) assay to detect the bacteria in the stool samples of 3,533 children aged less than 59 months from the Gambia, Mali, Kenya, and Bangladesh, with or without moderate-to-severe diarrhea (MSD). The authors conclude that the qPCR method may help overcome the limitations of current diagnostic and surveillance methods for detecting Shigella, and that acceptance of this new standard by the field would substantially increase the fraction of MSD cases that are attributable to Shigella.

Author: Lindsay B, Ochieng JB, Ikumapayi UN, et al.

Published: 2013

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(Located at jcm.asm.org)

Citation: Lindsay B, Ochieng JB, Ikumapayi UN, et al. Quantitative PCR for Detection of Shigella Improves Ascertainment of Shigella Burden in Children With Moderate-to-Severe Diarrhea in Low-Income Countries. Journal of Clinical Microbiology. 2013;51(6):1740-1746.

Resource types: Peer-reviewed journal

Diseases/vaccines: Shigellosis and enterotoxigenic Escherichia coli (ETEC)

Topics: Disease burden and surveillance, Disease/vaccine specific information

Regions: Africa, Asia