We describe the functional elements of a diagnostic instrument and disposable enteric card system under development that rapidly identifies and differentiates Shigella, E. coli, Campylobacter, and Salmonella in stool samples. These elements, currently realized as microfluidic subcircuits on individual cards, are: (1) pathogen capture and lysing, (2) nucleic acid capture, (3) on-chip rapid PCR, and (4) lateral flow detection of amplicons. Presented at the Solid State Sensor, Actuator and Microsystems Workshop, June 4, 2006; Hilton Head Island, SC.

Author: Weigl BH, Gerdes JC, Tarr P, et al

Publication date: June 2006

Region: Global

This poster reports progress incorporating magnetic beads within a unique lab card that permits efficient capture and enrichment of E. coli O157:H7 from feces. Presented at the 106th General Meeting of the American Society for Microbiology, May 21, 2006; Orlando, FL.

Author: Gerdes JC, Weigl BH, Yager P

Publication date: May 2006

Region: Global

This edition of PATH Today features the articles "A laboratory on a card," "Using microfluidic tests to identify infection," and "Common proteins for common prevention," which highlights the work of John Boslego and his team on pneumococcus. It also includes a letter from donor Shannon Scopa about her family's ordeal with rotavirus.

Publication date: May 2006

Region: Global

Part of series: PATH Today

An exploration of one technique for drying reagents onto a microfluidics card. Reagents needed for immunocapture, lysis, and PCR for pathogen capture can be stored in trehalose or trehalose dextran. Presented at the 1st Distributed Diagnosis and Home Healthcare Conference, April 2, 2006; Arlington, VA.

Author: Ramachandran S, Weigl BH, Gerdes JC, et al

Publication date: April 2006

Region: Global

Demonstration of the PATH enteric flow-through assay as a rapid and cost effective method for detecting pathogenic bacteria in stool samples in low-resource settings. Presented at the American Association for Clinical Chemistry Oak Ridge Annual Conference, April 17, 2006; San Jose, CA.

Author: Dillman L, Fichtenholtz A, Weigl B

Publication date: April 2006

Region: Global

The April 2005 issue of Directions in Global Health features five PATH projects: efforts to improve health among migrant communities in China, Thailand, and Cambodia; the Global Campaign for Microbicides, PATH's Woman's Condom, procurement of safe-injection supplies under the PEPFAR program, and the Rotavirus Vaccine Program.

Author: PATH

Publication date: April 2005

Region: Global

Part of series: Directions in Global Health

Publication date: September 2003

Region: Global

Part of series: PATH Today