This poster reports progress incorporating magnetic beads within a unique lab card that permits efficient capture and enrichment of E. coli O157:H7 from feces. Presented at the 106th General Meeting of the American Society for Microbiology, May 21, 2006; Orlando, FL.

Author: Gerdes JC, Weigl BH, Yager P

Publication date: May 2006

Region: Global

This edition of PATH Today features the articles "A laboratory on a card," "Using microfluidic tests to identify infection," and "Common proteins for common prevention," which highlights the work of John Boslego and his team on pneumococcus. It also includes a letter from donor Shannon Scopa about her family's ordeal with rotavirus.

Publication date: May 2006

Region: Global

Part of series: PATH Today

An exploration of one technique for drying reagents onto a microfluidics card. Reagents needed for immunocapture, lysis, and PCR for pathogen capture can be stored in trehalose or trehalose dextran. Presented at the 1st Distributed Diagnosis and Home Healthcare Conference, April 2, 2006; Arlington, VA.

Author: Ramachandran S, Weigl BH, Gerdes JC, et al

Publication date: April 2006

Region: Global

Demonstration of the PATH enteric flow-through assay as a rapid and cost effective method for detecting pathogenic bacteria in stool samples in low-resource settings. Presented at the American Association for Clinical Chemistry Oak Ridge Annual Conference, April 17, 2006; San Jose, CA.

Author: Dillman L, Fichtenholtz A, Weigl B

Publication date: April 2006

Region: Global

An overview of a method of sample preparation by smart polymers for detection in an immunoassay, demonstrated as part of a diagnostics test for Chlamydia trachomatis. Presented at: 38th Annual American Association of Clinical Chemistry (AACC) Oakridge Conference, April 21, 2006; San Jose, CA.

Author: Kulkarni S, Peck R, Barfield C, et al

Publication date: April 2006

Region: Global

Demonstration of the use of a magnetic immunochromatographic strip (ICS) assay with a reader to detect recombinant HPV-16 E6. The magnetic ICS assay was able to detect as little as 2.6ng of protein. Presented at: 38th Annual Oak Ridge Conference, April 20, 2006; San Jose, CA.

Author: Peck R, Schweizer J, Weigl B, et al

Publication date: April 2006

Region: Global

This document provides evidence from a study in Zimbabwe that demonstrates the correspondence between serum retinol and retionol-binding protein in estimating the prevalence of and screening for vitamin A deficiency.

Publication date: 2006

Region: Africa

This document summarizes available evidence for the validation and establishment of a rapid, field-based tool for the assessment of vitamin A deficiency using the retinol-binding protein immunoassay (RBP-EIA) test.

Publication date: 2006

Region: Global

This document assesses the correspondence between retinol and retinol-binding protein (RBP), as well as the stability of RBP subjected to varying temperature conditions over time, the effect of light, and the feasibility of using dried blood spots. The assessment is part of a larger project examining the use of the RBP enzyme immunoassay (RBP-EIA) to test for vitamin A deficiency.

Publication date: 2006

Region: Africa

This document shares data from Guinea-Bissau that provide validation of the retinol-binding protein enzyme immunoassay (RBP-EIA) test from serum samples, while also providing initial data on the feasibility of using dried blood samples as a specimen type for the assessment of vitamin A deficiency in the field.

Publication date: 2006

Region: Africa

The December 2005 issue of Directions in Global Health features five projects: PATH's Japanese encephalitis project, our collaboration with WHO on gender-based violence, a "lab-on-a-card" technology for detecting enteric disease, new HPV DNA tests for detecting cervical cancer, and an innovative behavior-change project that is helping prevent HIV in Eritrea.

Author: PATH

Publication date: December 2005

Region: Global

Part of series: Directions in Global Health

The August 2005 issue of Directions in Global Health features five PATH projects: our work with in- and out-of-school youth as part of the African Youth Alliance, a new test for vitamin A deficiency, HIV prevention among vulnerable groups in Georgia, cost analysis of rapid syphilis tests, and home-based care of people living with AIDS in South Africa.

Author: PATH

Publication date: August 2005

Region: Global

Part of series: Directions in Global Health

A report on the comparison of results from the PATH GC-check rapid test for gonococcal infection to the 16SrRNA PCR test conducted in a laboratory. Presented at: International Society for Sexually Transmitted Diseases Research (ISSTDR), July 10, 2005; Amsterdam, Netherlands.

Author: Michel A, Aïna G, Ndour M, et al

Publication date: July 2005

Region: Global

Historical profile on the PATH development and advancement of the HIV dipstick.

Publication date: June 2005

Region: North America and Europe

Historical profile on the PATH development and advancement of point-of-care diagnostic tests.

Publication date: June 2005

Region: North America and Europe

Demonstration of a new 48-well HPV DNA batch test showing analytical sensitivity to 5000 copies of HPV DNA and other test characteristics that may be suitable for use in low-resource settings. Presented at: 22nd International Papillomavirus Conference, April 30, 2005; Vancouver, BC.

Author: Eder P, Nazarenko I, Peck R, Weigl B, Sellors J, Lorincz A

Publication date: May 2005

Region: Global

This study provided the first evidence of the biological comparability between serum retinol levels estimated from venous blood and capillary blood. This critical observation provides empirical evidence that this important indicator of vitamin A status may be measured from capillary blood, which has important implications for nonclinical population-based research.

Author: Gorstein J; Dary O; Pongtorn; Shell-Duncan B; Quick T; Hix J; Kandavasee; Wasanwisut E

Publication date: 2005

Region: Asia

This document serves as a guide to phlebotomists and a training aid in the proper methods of obtaining blood samples for analysis by the retinol-binding protein enzyme immunoassay (RBP-EIA) used in testing for vitamin A deficiency.

Publication date: 2005

Region: Global

The development of a rapid and simple immunochromatographic strip test using the L7/L12 Neisseria gonorrhoeae, testing using clinical samples. Presented at: 15th Biennial Congress, International Society of Sexually Transmitted Diseases Research, July 27, 2003; Ottawa, Canada.

Author: Matsyshen D, Svanas G, Maynard J, Tam M, Burgess Hay DC

Publication date: July 2003

Region: Global

Publication date: 2003

Region: Global